Basic Cell Culture

A Practical Approach (Cloth) by J.m. Davis

Publisher: Oxford University Press, USA

Written in English
Published: Pages: 301 Downloads: 448
Share This

Subjects:

  • Cellular biology,
  • Zoology & animal sciences,
  • General,
  • Life Sciences - Zoology - General,
  • Science,
  • Cell Culture,
  • Cells, Cultured,
  • Cytological Techniques,
  • Laboratory manuals,
  • Science/Mathematics

Edition Notes

ContributionsJ. M. Davis (Editor)
The Physical Object
FormatHardcover
Number of Pages301
ID Numbers
Open LibraryOL9644440M
ISBN 100199634343
ISBN 109780199634347

Description of the book "Culture of Animal Cells: A Manual of Basic Technique": This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue. The book should also prove useful to the experienced worker when entering a new field, setting up a new laboratory, or as a source of reference on basic techniques. Suitable for: The aim of this book is to guide the newcomer progressively through all those areas which nowadays are basic to . This full e-book covers the following key items for your reproducibility;-Introduction to cell culture incubators. Important points for the installation of incubators. Important points for usage. Cleaning and decontamination methods. Basic care of cell culture incubators. Tips to minimize contamination. Care in the event of contamination. Cell Culture Incubators. Cell cultures require a strictly controlled environment in which to grow. Cell culture incubators are used routinely to provide the correct growth conditions, such as temperature, degree of humidity and CO 2 levels, in a controlled and stable manner. Generally, they can be set to run at temperatures in the range of 28°C (for insect cell lines) to 37°C (for mammalian.

Aspirate unused cell solution and discard used cultureware. Mark new flask with cell line, PD (population doubling; see Basic Cell Passage/Culture for how to calculate), split ratio (), initials, and date. Store flask in humidified incubator set to 37 o C and 5% CO 2, Clean up Biosafety Cabinet and return reagents/media to the fridge.   The most complete resource on the techniques, equipment, principles, and practices of animal cell culture Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue 4/5.

Basic Cell Culture by J.m. Davis Download PDF EPUB FB2

This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial.

Cell Culture Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types.

The intent of. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

This text is an indispensable resource for those in or entering the field, including academic research scientists Cited by: The topics include quality control of cell lines, cloning, specific cell types and their requirements, basic cell culture technique, primary culture, and the establishment and maintenance of cell lines."--SciTech Book News.

About the Author. John Davis is at Bio-Products Laboratory, Elstree, Hertfordshire, UK/5(5). The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time.

This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of.

cell culture research, have been added to this latest edition of the handbook. The handbook is intended as a guide rather than an in-depth text book of cell culture and you are encouraged to consult relevant specialised literature to obtain more detailed information.

Design and Equipment for the Cell Culture Laboratory Laboratory DesignFile Size: 2MB. This is the excellent book which describes basic in cell culture and advancement, application and technology together The attached pdf will gives you a brief outline for cell culture All the best.

Basic Cell and Molecular Biology. This book is a very comprehensive text for understanding cell biology. Topics covered includes: Details of Protein Structure, Bioenergetics, Enzyme Catalysis and Kinetics, Glycolysis, the Krebs Cycle and the Atkins Diet, Electron Transport, Oxidative Phosphorylation and Photosynthesis, DNA Structure, Chromosomes and Chromatin, Details of DNA Replication and.

cell culture Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists.

This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture.

It will also prove useful to the experienced worker entering a new field or setting up a new The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications 4/5(4). Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today.

Successful hPSC culture requires the recreation of the in vivo stem cell microenvironment, or “niche”, which includes growth factors, cell-to-cell interactions and cell to matrix adhesions. Unlike many cell types, hPSCs are grown in aggregates, or colonies, which helps create this niche.

Basic Cell Culture Protocols, 2nd ed. will surely follow in the footsteps of the first edition and, in the words of the reviewers, prove a still more "clear, easy-to-use well-sourced, and precise" work that belongs "in every cell culture laboratory.".

Basic cell culture techniques for consistent results. Gibco Cell Culture Basics is an introduction to cell culture, covering topics such as laboratory setup, safety, and aseptic technique.

You'll also find basic cell culture techniques and methods for passaging, freezing, and thawing cultured cells. The bulk of the material presented in this book assumes background knowledge of the principles and basic procedures of cell and tissue culture.

However, it is recognized that people enter a specialized field, such as tissue engineering, from many different disciplines and, for this reason, may not have had any formal training in cell culture.

Surachai Unchern Laboratory Practice on Caco-2 Cell Culture. 4 Basic characteristics of tissue culture Tissue culture Tissue culture is used as a generic term to include the in vitro cultivation of organs, tissues and cells. Originally, the term is not limited to animal cells, but includes the in.

Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments.

Product Information "Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today."--BOOK JACKET.

If you are planning to set up any functional cell culture facility, small- or large-scale, Basic Cell Culture could be very useful. The book has high practical and scientific value.

It contains details about how to design the laboratory, and describes not only cell culture techniques but also other basic skills, such as how to load and run an by: 1.

Cell Culture and Its Application covers the proceedings of the First International Cell Culture Congress Symposium, which focuses on how cell culture technology could impact on cell biology.

The symposium aims to establish facilities for the cultivation of mammalian cells, which in turn would hopefully enhance basic cell biology research.

Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.

Cell Culture Basic Course Content & Learning Objective. This course will provide basic knowledge and build upon your existing expertise, so that your daily cell culture work becomes safer and more efficient. Beginners to cell culture often get by with only a basic and limited knowledge acquired from copying a.

ISBN: OCLC Number: Description: xxi, pages: illustrations ; 24 cm. Contents: Cell culture laboratory / Caroline B. Wigley --Sterilization / Peter L.

Roberts --Culture media / T. Cartwright, G.P. Shah --Basic cell culture technique and the maintenance of cell lines / James A.

McAteer, John Davis --Primary culture and the. CELL CULTURE BASICS (PDF 62P) Progress in Stem Cell Transplantation. The book covers a wide range of issues related to new developments and innovations in cell-based therapies discussed in basic and clinical chapters from authors around the world involved in stem cell studies and research.

It thereby complements and extends the basic. Welcome to the companion site for the sixth edition of The Culture of Animal Cells by R.

Ian Freshney. On this site you will find: Figures from the book as Powerpoints for downloading A list of COAC suppliers ; A database of cross contaminated or misidentified cell lines; Resources are displayed in two ways.

Why is cell culture used for. Areas where cell culture technology is currently playing a major role. • Model systems for Studying basic cell biology, interactions between disease causing agents and cells, effects of drugs on cells, process and triggering of aging & nutritional studies • Toxicity testing Study the.

Basic Principles of Cell Culture. In book: Culture of Cells for Tissue Engineering, pp.1 - Basic cell-handling techniques are discussed, including choice of media, primary culture, and Author: Robert Ian Freshney.

Cell culture is a very versatile tool in the investigation of basic scientific and translation research questions. The advantage of using cell lines in scientific research is their homogeneity and associated reproducibility in data generated.

Plant Tissue Culture Terminology DifferentiatedCells that maintain, in culture, all or much of the specialized structure and function typical of the cell type in vivo.

Modifications of new cells to form tissues or organs with a specific function.Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment.

After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or medium that supplies.

Toni Lindl is Professor emeritus of the University of Applied Sciences in Weihenstephan, Germany, and CEO of the Institute of Applied Cell Culture Ltd., Munich.

After studies of biochemistry and cell biology he obtained his PhD in neurochemistry. Following post doc fellowships at the University of Constance and Bonn, Germany, he founded the Institute of Applied Cell Culture Ltd.

in and in.